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Characterisation and bioactivity of lentinan from Lentinula edodes.

by mickou@[EMAIL PROTECTED] (Sara) May 18, 2005 at 11:20 PM

Hi,

My name is Sara Ruiz Esparza Morales, I am a posgrade student in the
Centro de Investigación en Alimentación y Desarrollo in Hermosillo
Sonora, Mexico. I have been working during almost 2 years in
Biopolymers Lab, with a polysaccharide from the fungi Lentinula
edodes.

Fungi are an im****tant source of substances than functions from the
development of new drugs.  A variety of polysaccharides obtained from
fungi have the habiliy to behave as immunomodulators (enhacers the
immune system). The most active appear to be branched &#61538;(1,3) D
glucans with &#61538;(1,6)D glucopiranosyl units linked side chains.

One of the most studied bioactive b-glucans is named "lentinan", which
is isolated from Lentinus edodes. I am working in the characterization
of lentinan isolated from the fruiting body and mycelium of this
mushroom. I have used dynamic and static LS (light scattering) for the
determination of molecular weigth (MW), hydrodinamic radius (RH) and
radius of gyration (RG). The intrinsic viscosity was measured in a
Rheometer.

Now I am working with NMR spectroscopy for the determination or the
structure of the &#61538;-glucans and with metylathion and conversion
into PMAAs (partially methylated alditol acetates). The PMAAs will be
separated in a gas chromatographe.

Other part of my work is the study of the immunoproperties of the
B-glucans by measuring the activation of macrophages.

I will finish my postgrade in August so I have a short time for
getting the references I required in my study.  I would appreciate if
you have some information that could help me in my work.  If the
information that you could send me isn´t  very relevant for my
investigation, I would appreciate the same.

My mail : sararem@[EMAIL PROTECTED]
"Gracias".
 




 1 Posts in Topic:
Characterisation and bioactivity of lentinan from Lentinula edod
mickou@[EMAIL PROTECTED]   2005-05-18 23:20:07 

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